Pyruvate kinase isoenzymes: L-PK - liver foci

The rat was fed with a carcinogenic agent N-nitroso guanidine for 21 days and sacrified after four weeks without treatment.

L-PK staining of the liver

Counterstaining of M2-PK

L-PK staining of the liver

Counterstaining of M2-PK

L-PK negative preneoplastic foci appear.

Some of the L-PK negative preneoplastic foci are M2-PK positive.