Glycolytic enzyme complex: isolation

The glycolytic enzyme complex can be demonstrated by isoelectric focusing:
Proteins that are associated inside the glycolytic enzyme complex focuse at a common isoelectric point that is different from the isoelectric points of the purified individual proteins.

RNAse treatment leads to a disruption of the complex which is reflected by a dispersion of the isoelectric points of the individual proteins.


Transformation is correlated with migrations of proteins in- and outside the glycolytic enzyme complex that are reflected by shifts in the isoelectric points of the proteins.